This is a 1/10 dilution. Next, you would remove 1 ml from this dilution and add it to the second 9ml tube of buffer. This is also a 1/10 dilution, but not of the original sample. Instead, this is a 1/10 dilution of a 1/10 dilution. To arrive at the final dilution, multiply the second dilution by the first dilution: 1/10 X 1/10 = 1/100. V 1 = 10.00 mL = 10.00 mL ÷ 1000 mL/L = 10 × 10-3 L = 0.010 L (since there are 1000 mL in 1 L) c 2 = 0.20 mol L-1 V 2 = 100.00 mL = 100.00 mL ÷ 1000 mL/L= 100 × 10-3 = 0.10 L What is the relationship between what you know and what you need to find out? Write the dilution equation (dilution formula or dilution expression): Mar 29, 2019 · Perform the second dilution. For the second serial dilution, you will take 1 mL of solution from tube 1:10 and add it to the 9 mL of dilution liquid in the tube 1:100. Thoroughly mix tube 1:10 before adding to the next tube. Again, mix the tube 1:100 following dilution. The solution from test tube 1:10 has been diluted 10-fold into test tube 1:100.